* Block Type = 96-Well 0.2-mL Block																									
* Calibration Background is expired  = No																									
* Calibration Background performed on = 05-07-2015																									
* Calibration Pure Dye ABY is expired = No																									
* Calibration Pure Dye ABY performed on = 05-07-2015																									
* Calibration Pure Dye FAM is expired = No																									
* Calibration Pure Dye FAM performed on = 05-07-2015																									
* Calibration Pure Dye JUN is expired = No																									
* Calibration Pure Dye JUN performed on = 05-07-2015																									
* Calibration Pure Dye NED is expired = No																									
* Calibration Pure Dye NED performed on = 05-07-2015																									
* Calibration Pure Dye ROX is expired = No																									
* Calibration Pure Dye ROX performed on = 05-07-2015																									
* Calibration Pure Dye SYBR is expired = No																									
* Calibration Pure Dye SYBR performed on = 05-07-2015																									
* Calibration Pure Dye TAMRA is expired = No																									
* Calibration Pure Dye TAMRA performed on = 05-07-2015																									
* Calibration Pure Dye VIC is expired = No																									
* Calibration Pure Dye VIC performed on = 05-07-2015																									
* Calibration ROI is expired  = No																									
* Calibration ROI performed on = 05-07-2015																									
* Calibration Uniformity is expired  = No																									
* Calibration Uniformity performed on = 05-07-2015																									
* Chemistry = TAQMAN																									
* Date Created = 2015-07-21 15:40:17 PM PDT																									
* Experiment Barcode = NA																									
* Experiment Comment = NA																									
* Experiment File Name = F:\HF183_Env. Samples_07-21-15.eds																									
* Experiment Name = Quantification-TaqMan																									
* Experiment Run End Time = 2015-07-21 10:17:53 AM PDT																									
* Experiment Type = Standard Curve																									
* Instrument Name =       QS3																									
* Instrument Serial Number = 272320014																									
* Instrument Type = QuantStudioå¨ 3 System																									
* Passive Reference = ROX																									
* Quantification Cycle Method = Ct																									
* Signal Smoothing On = true																									
"* Stage/ Cycle where Analysis is performed = Stage 2, Step 2"																									
* User Name = NA																									
																									
[Results]																									
Well	Well Position	Omit	Sample Name	Target Name	Task	Reporter	Quencher	CT	Quantity	Quantity Mean	Quantity SD	Y-Intercept	R(superscript 2)	Slope	Efficiency	Automatic Ct Threshold	Ct Threshold	Automatic Baseline	Baseline Start	Baseline End	Amp Status	Comments	EXPFAIL	HIGHSD	NOAMP
1	A1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
2	A2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
3	A3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
4	A4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	19.215	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	16	AMP		N	Y	N
5	A5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	18.648	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	16	AMP		N	Y	N
6	A6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	18.506	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	16	AMP		N	Y	N
7	A7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.364	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
8	A8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.446	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
9	A9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.138	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
10	A10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.868	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
11	A11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	33.409	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	31	AMP		N	Y	N
12	A12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	33.351	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	31	AMP		N	Y	N
13	B1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
14	B2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	N
15	B3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
16	B4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	28.669	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	26	AMP		N	Y	N
17	B5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	28.79	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	26	AMP		N	Y	N
18	B6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	28.754	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	26	AMP		N	Y	N
19	B7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	29.741	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	27	AMP		N	Y	N
20	B8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	29.678	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	27	AMP		N	Y	N
21	B9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	30.521	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	28	AMP		N	Y	N
22	B10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	31.677	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
23	B11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.109	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
24	B12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.413	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
25	C1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
26	C2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
27	C3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
28	C4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
29	C5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
30	C6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
31	C7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	21.676	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	19	AMP		N	Y	N
32	C8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	21.485	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	19	AMP		N	Y	N
33	C9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	21.422	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
34	C10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	31.565	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
35	C11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.823	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
36	C12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	33.708	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	31	AMP		N	Y	N
37	D1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
38	D2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
39	D3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
40	D4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	25.259	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	22	AMP		N	Y	N
41	D5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	25.311	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	22	AMP		N	Y	N
42	D6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	25.554	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	23	AMP		N	Y	N
43	D7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	21.229	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	19	AMP		N	Y	N
44	D8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	21.354	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
45	D9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	21.261	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
46	D10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	31.338	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
47	D11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.331	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
48	D12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.663	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
49	E1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	34.712	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	32	AMP		N	Y	N
50	E2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	34.053	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	31	AMP		N	Y	N
51	E3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	35.851	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	33	AMP		N	Y	N
52	E4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.005	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
53	E5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.135	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
54	E6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	19.987	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
55	E7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	31.928	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
56	E8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.125	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
57	E9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.691	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
58	E10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
59	E11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
60	E12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
61	F1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	34.985	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	32	AMP		N	Y	N
62	F2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	33.414	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
63	F3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.923	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
64	F4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	19.935	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
65	F5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.221	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
66	F6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.201	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
67	F7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.172	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
68	F8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	31.345	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
69	F9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	31.345	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	29	AMP		N	Y	N
70	F10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	30.981	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	28	AMP		N	Y	N
71	F11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	30.962	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	28	AMP		N	Y	N
72	F12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	30.905	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	28	AMP		N	Y	N
73	G1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
74	G2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
75	G3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
76	G4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.097	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
77	G5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.139	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	17	AMP		N	Y	N
78	G6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.252	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
79	G7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
80	G8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
81	G9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
82	G10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
83	G11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
84	G12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
85	H1	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	26.026	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	23	AMP		N	Y	N
86	H2	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	25.817	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	22	AMP		N	Y	N
87	H3	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	25.743	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	23	AMP		N	Y	N
88	H4	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.529	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
89	H5	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.566	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
90	H6	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	20.475	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	18	AMP		N	Y	N
91	H7	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	32.729	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	30	AMP		N	Y	N
92	H8	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	33.479	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	31	AMP		N	Y	N
93	H9	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	34.295	1	1		0	0	0	-100	FALSE	0.03	TRUE	3	32	AMP		N	Y	N
94	H10	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	Y
95	H11	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	N
96	H12	FALSE	Sample 1	HF183	STANDARD	FAM	NFQ-MGB	Undetermined	1			0	0	0	-100	FALSE	0.03	TRUE	3	49	NOAMP		Y	N	N